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为了研究不同剂量绿萝花对大鼠组织器官抗氧化能力的影响,探讨绿萝花的抗衰老及毒性作用机理,试验将正常大鼠分为空白组、低剂量绿萝花组、中剂量绿萝花组、高剂量绿萝花组和大剂量绿萝花组,连续给药28 d,测定脏器的超氧化物歧化酶活性(SOD)、丙二醛含量(MDA)、总抗氧化能力(T-AOC)、单胺氧化酶活性(MAO)、谷胱甘肽过氧化氢酶活性(GSH-Px)、过氧化氢酶活性(CAT)。结果表明:连续服用绿萝花28 d后,低(1.0 g/d)、中(2.0 g/d)、高(4.0 g/d)剂量绿萝花能引起大脑、肝脏和心脏的SOD、T-AOC、GSH-Px、CAT活性上升,MDA含量下降,MAO活性下降;大剂量绿萝花(10.0 g/d)则引起大脑、肝脏和心脏的SOD、T-AOC、GSH-Px、CAT活性下降,MDA含量上升,MAO活性上升。说明绿萝花具有增强组织器官抗氧化能力的作用;抗氧化作用与剂量有关,大剂量绿萝花具有一定的毒性作用。
In order to study the effect of different doses of green flower on antioxidant capacity of tissues and organs of rats and to explore the anti-aging and toxic mechanism of green flower, the rats were divided into blank group, low dose green flower group, medium dose green flower group, High dose green flower group and high dose green flower group were administrated continuously for 28 days. SOD, MDA, T-AOC, MAO, GSH-Px and CAT. The results showed that the SOD, T-AOC contents of the brain, liver and heart were significantly decreased after a dose of 1.0 g / d, 2.0 g / d and 4.0 g / (P <0.05), the activity of GSH-Px, CAT increased, the content of MDA decreased and the activity of MAO decreased. The activity of SOD, T-AOC, GSH-Px and CAT in brain, liver and heart decreased The content increased, MAO activity increased. It shows that the green flower has the function of enhancing the antioxidant capacity of tissues and organs. The anti-oxidant effect is related to the dose. The green flower has a certain toxic effect.