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目的通过比较正常人与慢性乙肝、肝硬变和肝癌患者血清之间的拉曼光谱差异,探索基于表面增强拉曼散射(SERS)的血清诊断新方法。方法首先制备纳米银溶胶作为活性基底,分别测量30例正常人和48例慢性乙肝、48例肝硬变、46例肝癌患者血清的增强拉曼信号,并进行正交偏最小二乘法判别分析(OPLS-DA)。结果正常人与3组肝病患者血清在位移625、725、806、947、1018、1219、1131、1329、1440、1580、1660 cm-1处均有拉曼峰,且峰强弱存在差异;正常人血清在位移1096和1395 cm-1处有强峰,肝病患者血清在位移887 cm-1有强峰。ROC曲线下面积(AUC)分别为0.981、0.966、0.984。结论初步研究表明血清SERS图谱可作为肝病早期诊断的一种辅助手段。
Objective To explore a new serum diagnostic method based on surface enhanced Raman scattering (SERS) by comparing the Raman spectra of normal and chronic hepatitis B, cirrhosis and liver cancer patients. Methods Nanometer silver sol was prepared as the active substrate. The enhanced Raman signal of 30 normal individuals and 48 patients with chronic hepatitis B, 48 patients with liver cirrhosis and 46 patients with hepatocellular carcinoma was measured and analyzed by orthogonal partial least squares OPLS-DA). Results The serum of normal people and three groups of patients with liver disease had Raman peaks at 625,725,806,947,1018,1219,1131,1329,1440,1580,1660 cm-1, and the peak intensity was different; Human serum had strong peaks at displacements 1096 and 1395 cm-1, and sera from patients with liver disease had strong peaks at a displacement of 887 cm-1. The area under the ROC curve (AUC) was 0.981, 0.966, 0.984, respectively. Conclusion Preliminary studies have shown that serum SERS pattern can be used as an early aid in the diagnosis of liver disease.