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目的研究异体输血对急性淋巴细胞白血病(ALL)患儿白细胞介素-2(IL-2)、可溶性白介素2受体(sIL-2R)及前列腺素E2(PGE2)血清水平的影响,探讨异体输血的免疫抑制作用。方法采用ELISA方法检测接受不同输血剂量的ALL患儿在不同时间点血清中IL-2、sIL-2R与PGE2的水平。结果小剂量输血组(组1)与未输血组(组3)1个月时IL-2水平显著下降(P<0.05),但3个月时回升至输血前水平。大剂量输血组(组2)输血后IL-2水平持续下降,输血后3个月IL-2水平明显低于组1与组3(P分别<0.01,<0.05)。组1与组3的sIL-2R水平1个月时降低(P<0.01),并且在3个月时仍低于输血前水平(P<0.01);大剂量输血组(组2)sIL-2R水平于输血后1个月明显下降(P<0.01),输血后3个月回升至输血前水平。组1输血后PGE2水平持续降低(P<0.05),组2输血后1个月PGE2水平开始下降(P<0.05),在输血后3个月回升至输血前水平。结论大剂量输血使ALL患儿血清IL-2水平降低,sIL-2R和PGE2水平升高,而且这种变化在输血后3个月才能较明显表现出来。
Objective To investigate the effects of allogeneic blood transfusion on serum levels of interleukin-2, soluble interleukin 2 receptor (sIL-2R) and prostaglandin E2 (PGE2) in children with acute lymphoblastic leukemia (ALL) The immunosuppressive effect. Methods Serum levels of IL-2, sIL-2R and PGE2 were measured by ELISA in children with ALL at different transfusion doses at different time points. Results The level of IL-2 in the low-dose transfusion group (group 1) and non-transfusion group (group 3) decreased significantly (P <0.05) at 1 month, but returned to the level before transfusion at 3 months. The levels of IL-2 in high-dose transfusion group (group 2) decreased continuously after transfusion, and the level of IL-2 in transfusion group was significantly lower than that in group 1 and group 3 at 3 months after transfusion (P <0.01, <0.05). The levels of sIL-2R in group 1 and group 3 were lower at 1 month (P <0.01), and still lower than before transfusion at 3 months (P <0.01); sIL-2R Levels decreased significantly 1 month after transfusion (P <0.01), and returned to pre-transfusion levels 3 months after transfusion. The level of PGE2 decreased continuously (P <0.05) after transfusion in group 1 (P <0.05). The level of PGE2 decreased at 1 month after transfusion in group 2 (P <0.05), and returned to the level before transfusion 3 months after transfusion. Conclusion High-dose blood transfusion reduces serum IL-2 levels and sIL-2R and PGE2 levels in children with ALL, and this change can be clearly demonstrated 3 months after transfusion.