【摘 要】
：
As the open reading frames of hepatitis B virus (HBV) genomes are overlapping,resistance mutations (MTs) in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type (WT) HB
【机 构】
：
Department of Laboratory Medicine, Maternal and Child Health Hospital of Hubei Province, Tongji Medi
论文部分内容阅读
As the open reading frames of hepatitis B virus (HBV) genomes are overlapping,resistance mutations (MTs) in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type (WT) HBV.The question was raised how the coexistence of nucleos(t)ide analogs (NAs) resistance MTs and WT sequences affects HBV replication.In the present study,HBV genomes with frequently detected reverse transcriptase (RT)/surface truncation MTs,rtA181T/sW172*,rtV191I/sW182* and rtM204I/sW196*,were phenotypically characterized alone or together with their WT counterparts in different ratios by transient transfection in the absence or presence of NAs.In the absence of NAs,RT/surface truncation MTs impaired the expression and secretion of HBV surface proteins,and had a dose-dependent negative effect on WT HBV virion secretion.However,in the presence of NAs,coexistence of MTs with WT maintained viral replication,and the presence of WT was able to rescue the production of MT HBV virions.Our findings reveal that complementation of WT and MT HBV genomes is highly effective under drug treatment.
其他文献
Mosquitoes are classified into approximately 3500 species and further grouped into 41 genera.Epidemiologically,they are considered to be among the most important disease vectors in the world and they can harbor a wide variety of viruses.Several mosquito v
Coronaviruses are large, enveloped, positive-strand RNA viruses.Several coronaviruses are pathogenic in humans,including severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV) and this novel virus
Hepatitis E virus (HEV) infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farming areas in central China are still
An infection by Zika virus (ZIKV),a mosquito-borne flavivirus,broke out in South American regions in 2015,and recently showed a tendency of spreading to North America and even worldwide.ZIKV was first detected in 1947 and only 14 human infection cases wer
Tripartite motif (TRIM) proteins were shown to play an important role in innate antiviral immunity.FinTRIM (ftr) is a new subset of TRIM genes that do not possess obvious ortho1ogs in higher vertebrates.However,little is known about its function.In this s
Hepatitis B virus (HBV) is an important human pathogen belonging to the Hepadnaviridae family,Orthohepadnavirus genus.Over 240 million people are infected with HBV worldwide.The reverse transcription during its genome replication leads to low fidelity DNA
HIV-indeterminate Western blotting (WB) results are typically obtained in WB confirmatory assays,and the number of indeterminate samples may increase with the detection of HIV infections,which will present considerable challenges for the management of HIV
The phosphatidylserine-specific phospholipase A1 (PLA1A) is an essential host factor in hepatitis C virus (HCV) assembly.In this study,we mapped the E2,NS2 and NS5A involved in PLA1A interaction to their lumenal domains and membranous parts,through which
Foot-and-mouth disease virus (FMDV) can infect domestic and wild cloven-hoofed animals.The non-structural protein 3D plays an important role in FMDV replication and pathogenesis.However,the interaction partners of 3D,and the effects of those interactions
To date,29 distinct microRNAs (miRNAs) have been reported to be expressed during herpes simplex virus infections.Sequence analysis of mature herpes simplex virus-1 (HSV-1) miRNAs revealed five sets of miRNAs that are complementary to each other:miR-H6-5p/