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近年来应用双波长薄层层析扫描法定量,已有不少报道。此法由于可在薄层板上直接定量,较过去一些薄层层析定量的方法具有准确度好、灵敏度高、方法简便、快速等优点,适用于多组分物质和微量组分的含量测定。关于青蒿素的含量测定,有薄层层析-紫外分光光度法,柱层析-分光光度法,碘量法等。我所抗疟药组对青蒿素作过薄层层析比色法定量,在此基础上,我们进行了青蒿素的双波长薄层层析扫描法(以下简
In recent years, the application of dual-wavelength thin-layer chromatography scanning has been reported. This method can be directly quantified on thin plates. Compared with the previous methods of thin layer chromatography, it has the advantages of good accuracy, high sensitivity, simple and rapid method, etc. It is suitable for the determination of multi-component substances and trace components. . On the determination of artemisinin content, there are thin layer chromatography - ultraviolet spectrophotometry, column chromatography - spectrophotometry, iodometric method and so on. In our institute, the antimalarial drug group quantified artemisinin by TLC colorimetry. Based on this, we conducted a dual-wavelength thin-layer chromatography scan of artemisinin (the following