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目前,肌酐分析大多是利用肌酐能与苦味酸呈色性质(Jaffe氏反应)作定量测定。很久以来,人们就已发现有很多物质在同样条件下与苦味酸呈色,该法特异性不强。几十年来,人们一直在致力于提高方法特异性。高效液相色谱法(HPLC)问世后,人们企图利用其极高的分辨力将体液中肌酐与其它物质分开,来提高方法特异性。近年来HPLC迅速发展,出现了一系列高效、高容量、高压层析柱。1977年,Brown等氏首先运用薄壳型强酸性离子交换剂HS-pellionex-SCX柱分离体液中的肌酐,柱后联接流动苦味酸反应器和510mm检测器,提高了方法特异性。但是,柱的容量和柱效还不够理想,
At present, most of the creatinine analysis uses the creatinine and picric acid color rendering properties (Jaffe’s reaction) as a quantitative measure. For a long time, people have discovered that there are many substances that exhibit coloration with picric acid under the same conditions. The specificity of this method is not strong. For decades, people have been working to improve method specificity. After the introduction of high-performance liquid chromatography (HPLC), people attempted to improve the method specificity by using its extremely high resolution to separate creatinine from other substances in body fluids. In recent years, rapid development of HPLC has led to the emergence of a series of high-efficiency, high-capacity, high-pressure chromatography columns. In 1977, Brown et al. first used the thin-shell type strongly acidic ion exchanger HS-pellionex-SCX column to separate creatinine in body fluids, and connected the flow picric acid reactor and the 510mm detector after column to improve the method specificity. However, column capacity and column efficiency are still not ideal.