【摘 要】
：
采用免疫组化技术检测催乳素受体蛋白(PRLR)在绵羊卵巢中的表达定位,建立绵羊卵泡颗粒细胞体外培养体系,将颗粒细胞分成4个处理组,添加不同浓度催乳素(PRL):对照组(不添加PRL
【机 构】
：
河北农业大学动物科技学院,河北保定071000
论文部分内容阅读
采用免疫组化技术检测催乳素受体蛋白(PRLR)在绵羊卵巢中的表达定位,建立绵羊卵泡颗粒细胞体外培养体系,将颗粒细胞分成4个处理组,添加不同浓度催乳素(PRL):对照组(不添加PRL)、试验Ⅰ组(0.05 mg/L PRL)、试验Ⅱ组(0.50 mg/L PRL)、试验Ⅲ组(5.00 mg/L PRL),用ELISA法检测各组颗粒细胞雌激素(E2)和孕酮(P4)的分泌水平,荧光定量RT-PCR检测FSHR、LHR、PRLR、StAR、CYP11和CYP19等基因相对表达量.结果表明:在绵羊卵巢中,PRLR蛋白主要在卵泡的颗粒细胞和黄体细胞中表达;添加PRL抑制了E2的分泌、促进了P4分泌,其中Ⅱ组和Ⅲ组E2分泌水平显著低于对照组和Ⅰ组(P＜0.05);P4随着PRL添加浓度的升高而升高,各试验组P4分泌水平均显著高于对照组(P＜0.05).各试验组颗粒细胞FSHR、LHR、PRLR、CYP11、StAR的mRNA表达量均极显著高于对照组(P＜0.01),而试验组CYP19的表达量极显著低于对照组(P＜0.01).综上,PRL通过上调FSHR、LHR、PRLR、CYP11和StAR mRNA表达,下调CYP19 mRNA的表达,抑制绵羊卵巢颗粒细胞E2的分泌、促进P4的分泌.通过研究添加PRL对绵羊卵巢颗粒细胞E2和P4分泌及相关基因表达的影响,为探讨PRL对卵泡发育的调控机制奠定基础.
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