论文部分内容阅读
为明确普通小麦-华山新麦草易位系9020-17-25-6的抗条锈病基因及其遗传特点,利用中国条锈菌小种CYR29对9020-17-25-6、铭贤169及其杂交后代F1、F2、F3代进行苗期抗条锈性鉴定及遗传分析,选取48条RGAP引物和491对SSR引物对接种CYR29的F2代群体进行筛选,寻找与抗病基因连锁的分子标记。结果表明:9020-17-25-6对CYR29具有良好的抗条锈性,由1对显性基因独立控制,暂定名为Yr Hua9020。筛选到2个RGAP标记(M1和M2)和位于染色体3AS上的4个SSR标记(Xwmc11、Xwmc532、Xcfd79、Xgwm2)与Yr Hua9020连锁,与目的基因的遗传距离分别为6.9、9.5、17.8、12.2、7.2和17.8 c M。与已定位于3A染色体上的抗条锈病基因的比较研究表明,Yr Hua9020是一个与已知基因不同的新的抗条锈病基因。
In order to clarify the resistance gene and its genetic characteristics of the common wheat-Huashanna new wheat translocation line 9020-17-25-6, using the Chinese stripe rust races CYR29 against 9020-17-25-6, Mingxian 169 and its F1 hybrids F1, F2 and F3 were identified for their resistance to stripe rust at seedling stage and genetic analysis. 48 RGAP primers and 491 pairs of SSR primers were screened for the F2 population of CYR29 inoculation to find the molecular markers linked to the resistance gene. The results showed that 9020-17-25-6 had good stripe rust resistance against CYR29 and was independently controlled by a pair of dominant genes, tentatively named Yr Hua9020. The two RGAP markers (M1 and M2) and the four SSR markers (Xwmc11, Xwmc532, Xcfd79, Xgwm2) located on chromosome 3AS were linked to Yr Hua9020 and the genetic distances to the target genes were 6.9, 9.5, 17.8 and 12.2 , 7.2 and 17.8 cM. A comparative study with stripe rust resistance genes located on chromosome 3A showed that Yr Hua9020 is a new stripe rust resistance gene different from known genes.