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目的 通过观察雷公藤内酯醇诱导 Ｂ Ａ Ｌ Ｂ／ｃ 小鼠脾细胞凋亡与细胞活化程度的关系，探讨该化合物免疫抑制的作用机理。方法 以脾淋巴细胞为研究对象， Ｐ Ｈ Ａ Ｐ、 Ｐ Ｍ Ａ 和ｒｈ Ｉ Ｌ２ 为有丝分裂原活化淋巴细胞。结合荧光染色法， Ｄ Ｎ Ａ 凝胶电泳及 Ｄ Ｎ Ａ 片段测定等方法，检测雷公藤内酯醇诱导凋亡的作用。结果 （１） 雷公藤内酯醇能够造成活化的淋巴细胞凋亡，并呈浓度依赖性；（２） 雷公藤内酯醇不能使处于静止状态的脾淋巴细胞凋亡；（３） 雷公藤内酯醇诱导淋巴细胞凋亡的作用，与淋巴细胞的活化程度和增殖反应程度密切。结论 雷公藤内酯醇不能造成静止状态的淋巴细胞发生细胞凋亡，只能够诱导已活化淋巴细胞发生细胞凋亡，并以此影响机体的免疫功能。
OBJECTIVE: To investigate the mechanism of triptolide-induced immunosuppression induced by triptolide in B A L B / c mouse spleen cells and the degree of cell activation. Methods Splenic lymphocytes were used as research objects. P H AP, P M A and rh I L2 were mitogen-activated lymphocytes. Combined with fluorescence staining, D N A gel electrophoresis and D N A fragment determination and other methods to detect the triptolide-induced apoptosis. Results (1) Triptolide induced apoptosis in activated lymphocytes in a concentration-dependent manner. (2) Triptolide did not induce apoptosis of splenic lymphocytes at rest. (3) Triptolide-induced The role of lymphocyte apoptosis, and lymphocyte activation and proliferation of close response. Conclusion Triptolide can not induce apoptosis of lymphocytes at rest state, and can only induce apoptosis of activated lymphocytes and thus affect the immune function of the body.