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目的探讨汉族人群中晚期糖基化终产物受体(RAGE)基因G82S、-429T/C和-374T/A多态性与非糖尿病性冠心病及其严重程度的相关性。方法以冠状动脉造影证实的112例健康个体(正常对照组)和270例非糖尿病性冠心病患者(冠心病组)为研究对象进行病例对照研究,冠心病严重程度采用冠脉病变支数进行评估,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法检测RAGE基因G82S、-429T/C和-374T/A的多态性。结果汉族人群RAGE基因G82S、-429T/C和-374T/A多态性的分布与高加索人群有显著差异(均P<0.05),这三个多态性的基因型分布和等位基因频率在正常对照组和冠心病组之间差异无统计学意义(均P>0.05),在冠脉病变支数分组间差异也无统计学意义(均P>0.05)。结论 RAGE基因G82S、-429T/C和-374T/A多态性的分布具有种族差异,这三个多态性与汉族人群非糖尿病性冠心病及其严重程度无相关性。
Objective To investigate the association between the polymorphisms of GAGE, -429T / C and -374T / A of advanced glycation end products receptor (RAGE) and non-diabetic coronary heart disease in Han population. Methods One hundred and twelve healthy individuals (control group) and 270 non-diabetic coronary heart disease patients (coronary heart disease group) confirmed by coronary angiography were selected as the case-control study. The severity of coronary heart disease was evaluated by the number of coronary lesions The polymorphisms of RAGE gene G82S, -429T / C and -374T / A were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Results The distribution of G82S, -429T / C and -374T / A polymorphisms of RAGE gene in Han population were significantly different from those in Caucasian population (all P <0.05). The genotype distribution and allele frequency of RAGE gene in Han population were The difference between the normal control group and the coronary heart disease group was not statistically significant (all P> 0.05), but there was no significant difference between the coronary artery disease group (all P> 0.05). Conclusion There is a racial difference in the distribution of RAGE G82S, -429T / C and -374T / A polymorphisms. These three polymorphisms have no correlation with non-diabetic coronary heart disease and its severity in Han population.