从胎肝和肝癌组织中纯化的 GGTP,经4%—30% PAGE 鉴定氛明分成二部分,其中第一部分活力最强,经亲和层析柱吸附后,免疫动物获纯的抗血清.该部分 GGTP 经10% PAGE 显示一条带,其分子量为92kD,pI 值为4.2.但经 SDS-PAGE 则显示二条蛋白带,其分子量分别为64kD 和27kD.酶免疫抑制试验表明该抗血清对 GGTP Ⅱ,Ⅱ’具有抑制作用,双扩散免疫试验产生单一沉淀线,并互相融合在一起.说明二种组织来源的 GGTP 的免疫学特性相一致. GGTP purified from fetal liver and liver cancer tissue was identified by 4% -30% PAGE and divided into two parts, the first part of which was the most active and the pure animal antiserum was obtained after the affinity chromatography column was adsorbed. Some GGTP showed a band with 10% PAGE, its molecular weight was 92kD and pI value was 4.2, but SDS-PAGE showed two bands with molecular weights of 64kD and 27kD, respectively. Enzyme-linked immunosorbent assay showed that the antiserum against GGTPII , Ⅱ ’, double diffusion immunoassay produced a single precipitation line, and merged with each other, indicating that the immunological characteristics of GGTP derived from the two tissues are consistent.