论文部分内容阅读
目的 研究添加生长激素 (rhGH)及谷氨酰胺 (Gln)的肠外营养 (PN)对短肠大鼠残存小肠代偿的作用及机制。方法 按 2× 2析因实验方案 ,将SD大鼠随机分成STD、Gln、rhGH及rhGH +Gln组 ,建立PN短肠动物模型。PN 6d后行小肠黏膜形态学检查 ,并行细胞增殖核心抗原 (PCNA)测定、原位末端标记 (TUNEL)染色及胰岛素样生长因子 1(IGF 1)mRNA的Northernblot测定。结果 rhGH+Gln组残余小肠黏膜形态学上呈显著代偿表现。析因分析表明 ,rhGH与Gln间存在协同作用 (P <0 .0 1)。其PCNA表达显著高于rhGH、Gln与STD组 ,分别为 2 4.95± 3 .93、19.2 8± 3 .2 5、17.2 7± 3 .38与8.37± 2 .2 3(P <0 .0 1) ;凋亡指数显著降低 ,分别为 5 .6 8± 2 .0 7、8.0 6± 2 .33、10 .0 0± 2 .2 4及 2 2 .32±3.84(P <0 .0 1) ;小肠IGF 1mRNA表达在rhGH +Gln组显著高于rhGH、Gln及STD组 ,分别为 0 .73±0 .0 5、0 .6 2± 0 .0 4、0 .5 1± 0 .0 4及 0 .41± 0 .2 2 (P <0 .0 5 )。结论 rhGH与Gln通过促进肠黏膜上皮细胞增生与抑制其凋亡 ,协同促进短肠大鼠残存小肠代偿 ,小肠IGF 1在二者协同作用的发挥中起重要的介导作用
Objective To study the effect and mechanism of parenteral nutrition (PN) supplemented with rhGH and glutamine on residual intestinal compensatory of short-term rats. Methods According to 2 × 2 factorial experimental protocol, SD rats were randomly divided into STD, Gln, rhGH and rhGH + Gln groups. Small intestine mucosa morphological examination was performed after PN 6d. Parallel cell proliferation core antigen (PCNA) assay, TUNEL staining and Northern blot analysis of insulin-like growth factor 1 (IGF-1) mRNA were performed. Results The remnants of small intestinal mucosa in rhGH + Gln group showed significant compensatory changes. Factorial analysis revealed a synergistic effect between rhGH and Gln (P <0.01). The expression of PCNA was significantly higher than that of rhGH, Gln and STD groups (2 4.95 ± 3.93, 19.2 8 ± 3.25, 17.2 7 ± 3.38 and 8.37 ± 2.23 (P <0.01 ), The apoptotic index decreased significantly, which were respectively 5.68 ± 2.0.7, 8.06 ± 2.33, 10.00 ± 2.24 and 2.32 ± 3.84 (P <0.01 ); IGF 1 mRNA expression in small intestine in rhGH + Gln group was significantly higher than that in rhGH, Gln and STD groups, which were 0 .73 ± 0 .0 5,0 .62 ± 0 .0 4,0 .5 1 ± 0 .0 4 and 0 .41 ± 0 .2 2 (P <0. 05). Conclusions rhGH and Gln play an important role in promoting intestinal mucosal epithelial cell proliferation and inhibiting apoptosis, synergistically promoting the survival of small intestine in short-bowel rats. Intestinal IGF 1 plays an important role in the synergistic effect of both