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30例人鼻咽癌组织DNA和1例人鼻咽癌组织移植于裸鼠的肿瘤DNA,分别经酶切后与EBV—“W”片段(BamHI)探针作分子杂交。以pstⅠ酶切后,发现人鼻咽癌组织DNA、移植瘤DNA及Raji细胞DNA中呈现几乎完全一致的杂交图谱类型。出现6.1、2.8、2.1和1.0kb的四条带。30例人鼻咽癌组织中有25例(80.3％)含有EB病毒基因组。对上述阳性的DNA样品,经用BamHI酶切后,再杂交出巩6.7。3.1和2.0kb几乎完全相似的三条特征性杂交带,而各1例正常胎鼻组织、肝癌和乳腺癌組织DNA均不能和EBV—“W”片段杂交。此结果提示在人鼻咽癌组织中存在BEV的基因组,从杂交结果存在一定的规律性来看,EBV致鼻咽癌可能存在着严格的条件控制。
30 cases of human nasopharyngeal carcinoma DNA and 1 case of human nasopharyngeal carcinoma tissue were transplanted into the tumor DNA of nude mice, which were digested with EBV-“W” fragment (BamHI) probe for molecular hybridization. After digested with pstI, it was found that the DNA of human nasopharyngeal carcinoma, DNA of transplanted tumor and DNA of Raji cells showed almost identical patterns of hybridization patterns. There are four bands of 6.1, 2.8, 2.1 and 1.0 kb. Twenty-five (80.3%) of the 30 human nasopharyngeal carcinoma tissues contained the EBV genome. The positive DNA samples were digested with BamHI and then hybridized to three characteristic hybridization bands with almost identical 6.7, 3.1 and 2.0 kb, and one normal nasal tissue, liver cancer and breast cancer. Cannot hybridize with EBV-“W” fragment. This result suggests that there is a BEV genome in human nasopharyngeal carcinoma. From the point of view of the regularity of the hybridization results, there may be strict conditional control of EBV-induced nasopharyngeal carcinoma.