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目的探讨Olig在少突胶质细胞介导的脱髓鞘大鼠髓鞘再生中的作用。方法 40只健康Wistar大鼠,随机分成正常组、模型对照组、模型组、实验组,用形态学观察和免疫组织化学检测Olig1和Olig2的表达。结果正常组Olig1位于少突胶质细胞的胞质,模型组胞核表达明显增多,实验组Oligl又重新转移至胞质;正常组Olig2表达位于胞核,模型组中有少量表达于胞质。结论在Olig1和Olig2同时缺失时,导致全脑不能形成少突胶质细胞,严重影响髓鞘的再生。
Objective To investigate the role of Olig in the remyelination of oligodendrocyte-mediated demyelination in rats. Methods Forty healthy Wistar rats were randomly divided into normal group, model control group, model group and experimental group. Olig1 and Olig2 expression were detected by morphological observation and immunohistochemistry. Results Olig1 in the normal group was localized in the cytoplasm of oligodendrocytes. The expression of Olig1 in the model group was significantly increased. The expression of Olig2 in the normal group was localized in the nucleus. A small amount of Olig2 was expressed in the cytoplasm of the model group. Conclusions Olig1 and Olig2 simultaneously deleted, resulting in the whole brain can not form oligodendrocytes, seriously affecting myelin regeneration.