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本文采用组培愈伤组织接种法研究了不同抗病类型的杨树品种受溃疡病菌(Dothiorellagregaria Sacc.)侵染后苯丙氨酸解氨酶活性以及绿原酸和木质素含量的变化与抗病性的关系。纳果发现,受病菌侵染后各品种的苯丙氨酸解氮酶活性都有一个明显消长过程,但抗病品种的酶活性升高的幅度大(为感病品种的2倍),持续的时间也长;伴随酶活性的变化,各品种的绿原酸含量也发生明显变化,与感病品种相比,抗病品种的绿原酸不仅出现得早,而且积累的量也大(为感病品种的三倍);木质素的增量也与品种的抗病性呈正相关。上述结果表明,杨树对溃疡病的抗性与苯丙氨酸解氨酶的活性密切相关,该酶在抗病中的作用是通过其控制的代谢产物得以以表达。
In this paper, tissue culture callus inoculation method was used to study the changes of phenylalanine ammonia-lyase (ALA) activity and chlorogenic acid and lignin content in poplar varieties with different disease resistance types after inoculation with Dothiorellagregaria Sacc. Sick relationship. The results showed that there was a significant process of phenylalanine ammonia-lyase activity in all cultivars infected by pathogenic bacteria, but the activity of resistant cultivars increased by a large margin (twice that of susceptible cultivars) The chlorogenic acid content of all cultivars also changed significantly with the change of enzyme activity. Compared with the susceptible varieties, chlorogenic acid of resistant varieties not only appeared earlier, but also accumulated in large amount ( Three times the susceptible varieties); lignin increment also with the variety of disease resistance was positively correlated. The above results indicate that poplar’s resistance to ulcer disease is closely related to the activity of phenylalanine ammonia-lyase, and its role in disease resistance is expressed through its controlled metabolites.