论文部分内容阅读
目的通过检测模型犬邻近拔牙创微种植体骨界面与骨重建密切相关的生长因子——转化生长因子-β(TGF-β)、肿瘤坏死因子-α(TNF-α),骨钙素(OC)和核心结合因子基因(Cbfa1)等的表达,探讨拔牙创愈合对邻近微种植体骨界面生长因子的影响。方法 12只18月龄健康雄性比格犬,采取自身对照,建立邻近拔牙创植入微种植体实验动物模型(实验组),以非拔牙处植入微种植体为对照(对照组),分别于第1、3、8、12周依次处死,获得各带微种植体的组织块,采用免疫组织化学和原位杂交法,检测TGF-β、TNF-α、OC蛋白和Cbfa1基因表达,并对其表达结果进行半定量分析。结果实验组TGF-β、TNF-α蛋白以及Cbfa1基因表达在各检测时段均强于对照组,与对照组比较,1周时TNF-α的表达,3周时TGF-β、TNF-α的表达差异均具有统计学意义(P<0.05),8周时Cbfa1基因的表达差异具有统计学意义(P<0.05);OC蛋白的表达两组均随着术后时间的延长表达增强,术后8周达高峰,术后3周时实验组表达弱于对照组,差异有统计学意义(P<0.05)。结论拔牙创邻近微种植体植入后早期炎症反应较重,但持续时间不长。骨界面骨改建进程随着愈合时间的增加持续活跃,过长的愈合时间会使骨形成相关因子表达削弱,在整个过程中种植体能保持稳定。
OBJECTIVE: To detect the expression of TGF-β, TNF-α, osteocalcin (OC) and osteocalcin (OC), which are closely related to bone remodeling, ) And corebinding factor gene (Cbfa1), to explore the effect of tooth healing on the bone micro-interfacial growth factor. Methods 12 healthy male Beagle dogs aged 18 months were enrolled in this study. The control group was established by implanting micro-implants in the experimental group (experimental group), and the micro-implants were implanted in the control group (control group) The rats were sacrificed on the 1st, 3rd, 8th and 12th week respectively to obtain the tissue explants with micro-implants. The expressions of TGF-β, TNF-α, OC and Cbfa1 gene were detected by immunohistochemistry and in situ hybridization Semi-quantitative analysis of the expression results. Results Compared with the control group, the expression of TGF-β, TNF-α and Cbfa1 in experimental group were significantly higher than those in control group at 1 week (P <0.05). The expression of Cbfa1 gene at 8 weeks had statistical significance (P <0.05). The expression of OC protein increased with the extension of time after operation The peak was on the 8th week, and the expression in the experimental group was weaker than that in the control group at 3 weeks after operation, the difference was statistically significant (P <0.05). Conclusion The early microinjection of proximal micro-implants has a more severe inflammatory response after tooth extraction, but the duration is not long. The process of bone interface reconstruction continued to increase with the healing time. Excessive healing time impaired the expression of bone formation-related factors, and the implant remained stable throughout the procedure.