论文部分内容阅读
目的研究2002-2008年杭州市甲型副伤寒疫情分离株的亲缘性,并探讨脉冲场凝胶电泳(PFGE)、多位点序列分型(MLST)在甲型副伤寒沙门菌分子分型中的应用。方法对404株甲型副伤寒沙门菌运用脉冲场凝胶电泳(PFGE)进行分子分型,并用K-B法测定菌株抗生素敏感性。挑选9株甲型副伤寒沙门菌进行多位点序列分型(MLST)以及稳定性试验。结果 404株甲型副伤寒沙门菌可分为6个PFGE型和4个耐药型,P1型和P2型属于同一个克隆系,该克隆系菌株占试验菌株数的99%,分离自临安市的310株菌均为P1型,分离自杭州市西湖区的主要菌型是P2型和P1型。9株甲型副伤寒沙门菌根据SucA位点的差异可分为2个MLST型。结论杭州市2002-2008年的甲型副伤寒疫情由同一克隆系的菌株主导。PFGE、MLST分型方法各有侧重,可互为补充。
Objective To study the relationship between isolates of Parainhala Parainonitis A (Parain Paratyphi A) from Hangzhou in 2002-2008 and to investigate the effect of PFGE and MLST on the molecular typing of Salmonella paratyphi A Applications. Methods 404 Salmonella paratyphi A strains were genotyped by pulsed-field gel electrophoresis (PFGE), and their antibiotic susceptibilities were determined by K-B method. Nine strains of Salmonella paratyphi A were selected for multilocus sequence typing (MLST) and stability test. Results 404 strains of Salmonella paratyphi A were divided into 6 PFGE and 4 resistant strains, while P1 and P2 belonged to the same clonal line. The clone strains accounted for 99% of the tested strains, which were isolated from Lin’an Of the 310 strains are P1 type, isolated from Hangzhou West Lake District, the main bacteria are P2 type and P1 type. Nine strains of Salmonella paratyphi A can be divided into two MLST types according to the difference of SucA loci. Conclusion The epidemic of paratyphoid A in Hangzhou from 2002 to 2008 was dominated by the same clonal strain. PFGE, MLST classification methods have their own focus, can complement each other.