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目的检测乙型肝炎患者血清可溶性B7-H3(s B7-H3)和γ干扰素(IFN-γ)的水平,并探讨其临床意义。方法选取87例乙型肝炎患者,包括急性肝炎16例、慢性中至重度肝炎25例、肝硬化代偿期24例和肝硬化失代偿期22例,同期24例体检健康者为正常对照。采用ELISA检测乙型肝炎患者和对照人群血清s B7-H3和IFN-γ的含量,分析二者分别与天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、甲胎蛋白(AFP)、白细胞(WBC)计数及HBV DNA定量结果的相关性。结果正常对照组血清s B7-H3、IFN-γ水平为(27.82±12.75)ng/m L、(328.84±240.20)pg/m L,乙型肝炎患者血清s B7-H3、IFN-γ含量分别为(202.17±58.14)ng/m L和(3436.11±1605.01)pg/m L,均显著高于正常对照组。s B7-H3与IFN-γ密切相关,AST、ALT、AFP、WBC及HBV DNA与二者均无相关性。s B7-H3在急性肝炎时水平最高,IFN-γ则在慢性肝炎时水平最高。结论不同临床类型乙型肝炎患者的s B7-H3、IFN-γ含量明显高于正常者,连续监测血清s B7-H3、IFN-γ可能对乙型肝炎患者的疾病转归预测有重要临床价值。
Objective To detect the levels of serum soluble B7-H3 (s B7-H3) and interferon-γ (IFN-γ) in patients with hepatitis B and to investigate its clinical significance. Methods Totally 87 patients with hepatitis B were selected, including 16 acute hepatitis, 25 chronic moderate to severe hepatitis, 24 cirrhosis and 22 decompensated liver cirrhosis. 24 healthy subjects were normal controls. Serum levels of B7-H3 and IFN-γ in patients with hepatitis B and control were detected by ELISA. The levels of serum B7-H3 and IFN-γ in serum of patients with hepatitis B were compared with those of aspartate aminotransferase (AST), alanine aminotransferase (ALT) Protein (AFP), white blood cell (WBC) count and HBV DNA quantitative results. Results Serum levels of sB7-H3 and IFN-γ in normal control group were (27.82 ± 12.75) ng / m L, (328.84 ± 240.20) pg / m L, respectively. (202.17 ± 58.14) ng / m L and (3436.11 ± 1605.01) pg / m L, both significantly higher than the normal control group. s B7-H3 is closely related to IFN-γ, and there is no correlation between AST, ALT, AFP, WBC and HBV DNA. s B7-H3 is highest in acute hepatitis and IFN-γ is highest in chronic hepatitis. Conclusions The levels of s B7-H3 and IFN-γ in patients with different clinical types of hepatitis B were significantly higher than those in normal subjects. Continuous monitoring of serum levels of sB7-H3 and IFN-γ may have important clinical value in predicting the prognosis of patients with hepatitis B .