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目的探究我国HIV-1感染者T细胞分泌TGF-β和IL-10的水平,以及重组IL-10(r IL-10)和重组TGF-β(r TGF-β)对T细胞功能的影响。方法分离HIV-1感染者PBMC,加入r IL-2、r IL-15、r IL-12细胞因子体外刺激,采用胞内染色、流式细胞术检测T细胞内TGF-β、IL-10的产生情况。结果 HIV-1感染者总T细胞和CD8~+T细胞分泌的TGF-β明显高于正常人(P=0.000 1,P=0.003),分泌的IL-10在HIV-1感染者和正常人之间没有统计学差异;总T细胞分泌的TGF-β、IL-10与CD8~+T细胞分泌的TGF-β、IL-10有明显的正相关和正相关趋势(r=0.758,P=0.000 1;r=0.346,P=0.056);体外实验发现,r IL-10或r TGF-β对T细胞CD107a或IFN-γ的分泌具有抑制作用。结论 HIV-1感染者T细胞分泌的抑制性细胞因子TGF-β水平增加,抑制T细胞功能的发挥,因此在临床抗病毒治疗中,应注意调节细胞因子的平衡,降低TGF-β的抑制作用。
Objective To investigate the levels of TGF-β and IL-10 secreted by T cells from HIV-1 infected patients in China and the effects of recombinant IL-10 (r IL-10) and recombinant TGF-β (r TGF-β) on T cell function. Methods The PBMC of HIV-1 infected patients were isolated and stimulated with rIL-2, rIL-15 and rIL-12 cytokines in vitro. The expression of TGF-β and IL-10 in T cells was detected by flow cytometry Produce the situation. Results The levels of TGF-β secreted by total T cells and CD8 + T cells in HIV-1 infected patients were significantly higher than those in normal controls (P = 0.000 1, P = 0.003) There was no significant difference between the two groups (P> 0.05). The levels of TGF-β, IL-10 secreted by total T cells and TGF-β and IL-10 secreted by CD8 ~ + T cells were positively correlated and positively correlated 1; r = 0.346, P = 0.056). In vitro experiments showed that rIL-10 or r TGF-β could inhibit the secretion of CD107a or IFN-γin T cells. CONCLUSION: T-cells secreted by HIV-1 infected T cells may increase the level of TGF-β and inhibit the function of T cells. Therefore, in clinical anti-viral therapy, the balance of cytokines should be regulated and the inhibitory effect of TGF-β be reduced .