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作者研究了具有不同药理性质的40种药物在氰化钠引起的缺氧情况下,对体外培养的鸡胚胎脑神经细胞中ATP和蛋白质含量的作用,以测定细胞损伤和修复的程度、了解药物对细胞生存能力和代谢活性的影响,从而评价该方法的实用性。七天龄鸡胚胎脑半球神经细胞接种子多聚赖氨酸包被的培养瓶中,用含20％胎牛血
The authors investigated the effect of 40 drugs with different pharmacological properties on ATP and protein content in cultured chicken embryonic brain neurons in the absence of sodium cyanide-induced hypoxia to determine the degree of cellular damage and repair and to understand the effects of drugs On cell viability and metabolic activity in order to evaluate the practicality of the method. Seven-day-old chicken embryonic cerebral hemispheric neurons were seeded with polylysine coated flasks, with 20% fetal bovine serum