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研究低剂量重离子束预辐照对小鼠肝脏辐射损伤程度的影响。分别用低剂量12C6+离子束全身均匀预辐照处理小鼠,剂量分别为0、0.05、0.1、0.25、0.5Gy,剂量率为1Gy/min,4h后用4Gy的12C6+离子束全身均匀辐照,照射8h后用流式细胞仪检测辐照小鼠肝脏细胞在各细胞周期时相的百分率,并用单细胞电泳技术检测辐照损伤小鼠肝脏细胞的DNA损伤程度。结果显示,和对照组相比,低剂量预辐射处理可以减轻辐照损伤小鼠肝脏细胞G0/G1期和G2/M的阻滞,促进肝脏细胞在S期的积累。此外,辐照小鼠肝脏细胞的拖尾率及拖尾长度也显著减少,其中以0.1Gy处理组效果最为显著(P<0.01)。提示:低剂量重离子预辐照能使细胞产生适应性反应,有效减轻辐照小鼠肝脏细胞G0/G1期和G2/M的阻滞,并显著减轻肝脏细胞DNA的辐射损伤程度。
To study the effect of low dose heavy ion beam pre-irradiation on liver injury in mice. The mice were pretreated with low dose 12C6 + ion beam, and the dose was 0,0.05,0.1,0.25,0.5Gy, the dose rate was 1Gy / min. After 4h, the whole body was irradiated with 4Gy 12C6 + ion beam, 8h after irradiation, the percentage of liver cells in each cell cycle was detected by flow cytometry. The DNA damage of irradiated liver cells was detected by single cell electrophoresis. The results showed that, compared with the control group, low-dose pre-irradiation could reduce the arrest of G0 / G1 phase and G2 / M of liver cells in irradiated mice and promote the accumulation of liver cells in S phase. In addition, the tailing rate and trailing length of irradiated liver cells were also significantly reduced, of which 0.1Gy treatment group was the most significant (P <0.01). Hypothesis: Pretreatment with low dose of heavy ions can make the cells produce adaptive response, effectively reduce the G0 / G1 phase and G2 / M block of irradiated mouse liver cells, and significantly reduce the DNA damage of liver cells.