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目的:建立一种快速,准确,自动化的光纤传感μSI-LOV(顺序注射-阀上实验室)测定缬沙坦胶囊含量的新方法。方法:建立光纤传感μSI-LOV系统,在丙酮溶剂中氯冉酸与缬沙坦发生专属荷移,屏蔽辅料干扰。通过系统编程,优化实验条件,自动化测定缬沙坦胶囊的含量。结果:优选条件:吸入顺序依次为丙酮、缬沙坦、氯冉酸、缬沙坦及丙酮,缬沙坦前后吸入体积比为3:2,缬沙坦总吸入体积为200μL,氯冉酸吸入体积为20μL,缬沙坦的荷移吸光度与质量浓度在0.064-0.32mg/mL范围内的线性关系为A=1.497c+0.302,r=0.9998,精密度RSD为0.337%,回收率96.2%-102.9%,平均每粒含量为81.10 mg,占标示量101.27%,与HPLC法比较,t检验,P>0.05。结论:本方法通过制定个性化编程,实现自动化快速分析,重现性好,试剂用量少,分析效率高,成本低,方法可靠。
OBJECTIVE: To establish a new method for the determination of valsartan capsule content by μSI-LOV (Sequential Injection-Valve Laboratory) with fast, accurate and automatic optical fiber sensing. Methods: The optical sensing μSI-LOV system was established. Chlorpropion acid and valsartan were exclusively loaded in acetone solvent, and the shielding materials were interfered. Through the system programming, optimize the experimental conditions, automated determination of valsartan capsule content. Results: The optimal conditions were as follows: acetone, valsartan, chlorambucil, valsartan and acetone, the volume ratio of inhaled valsartan was 3: 2, the total inhaled volume of valsartan was 200μL, The volume of 20μL, the linear relationship between the absorbance and the mass concentration of Valsartan in the range of 0.064-0.32mg / mL was A = 1.497c + 0.302, r = 0.9998, the RSD of precision was 0.337% and the recovery was 96.2% 102.9%, the average content of 81.10 mg per tablet, accounting for 101.27% of the labeled amount, compared with the HPLC method, t test, P> 0.05. Conclusion: This method is through the development of personalized programming, rapid automated analysis, good reproducibility, less reagent, high efficiency, low cost, reliable method.