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以灰葡萄孢菌(Botrytis cinerea Pers.)为指示菌,采用单因素试验与正交实验相结合的方式,对TB-12菌株发酵培养基组成及培养条件进行优化,以提高番茄灰霉病拮抗菌Bacillus velezensis TB-12菌株的抑菌效果,并通过盆栽试验考察了TB-12菌株对番茄灰霉病的防治效果,为今后该菌剂生产工艺条件的进一步确定及其防治应用的开展奠定相应的基础。结果表明:TB-12菌株的最佳发酵培养基组合为乳糖2%,酪蛋白胨4%,MgSO_4·7H_2O 0.1%,KCl 0.05%;最佳培养条件为接种量10%,装瓶量50 mL,初始pH 8,发酵时间60h,培养温度30℃,摇床转速180r·min~(-1)。与采用基础发酵培养基相比,优化后TB-12菌株对灰葡萄孢菌的抑制效果增加了69.4%。盆栽试验表明,优化后TB-12菌株发酵上清液对番茄灰霉病的预防效果达到69.2%,治疗效果达到58.2%,分别提高了32.6%和19.5%。TB-12菌株对番茄灰霉病有良好的防治效果。
Using Botrytis cinerea Pers. As indicator, the fermentation medium composition and culture conditions of TB-12 strain were optimized by single factor experiment and orthogonal experiment to enhance the antagonism of tomato gray mold Bacillus velezensis TB-12 strain was tested for its bacteriostatic effect. The pot experiment was conducted to study the control effect of TB-12 against tomato gray mold. The results of this study indicated that the TB-12 strain could be used for further control of Botrytis cinerea. Foundation. The results showed that the best fermentation medium for TB-12 was 2% of lactose, 4% of casein peptone, 0.1% of MgSO 4 · 7H 2 O and 0.05% of KCl. The best culture conditions were 10% Initial pH 8, fermentation time 60h, culture temperature 30 ℃, shaking speed 180r · min ~ (-1). Compared with the basic fermentation medium, the inhibitory effect of the optimized TB-12 strain on Botrytis cinerea increased by 69.4%. Pot experiments showed that the optimized supernatant of TB-12 strain could prevent 69.9% of Botrytis cinerea. The treatment effect was 58.2%, which was increased by 32.6% and 19.5% respectively. TB-12 strain of tomato gray mold has a good control effect.