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目的:防治支原体污染是细胞培养中难以解决的问题.我们用CIP和BMcyclin联合治疗清除了杂交瘤细胞HAb18,A10,骨髓瘤细胞SP2/0和人白血病细胞K562中的支原体污染.方法:3株小鼠细胞系(HAb18,A10,SP2/0)用含有10μg/mlCIP的培养液治疗12d后根除了污染的支原体.人细胞株(K562)用CIP加BMcyclin联合治疗26d后清除了支原体的污染.结果:清除支原体后,细胞的增殖率和杂交瘤细胞抗体分泌稳定性增强.未见有任何毒性作用.结论:用CIP和BMcyclin清除培养细胞中支原体的污染是一个安全有效的方法.
Objective: Prevention and treatment of mycoplasma contamination is difficult to solve in cell culture. Our co-treatment with CIP and BMcyclin cleared mycoplasma contamination in hybridoma cells HAb18, A10, myeloma cells SP2 / 0 and human leukemia cells K562. Methods: Three strains of mouse cell lines (HAb18, A10, SP2 / 0) were treated with 10μg / ml CIP for 12 days to eradicate the contaminated Mycoplasma. Mycobacterial contamination was cleared from the human cell line (K562) treated with CIP plus BMcyclin for 26 days. Results: Mycoplasma clearance increased the cell proliferation rate and hybridoma cell antibody secretion stability. Did not see any toxic effects. Conclusion: It is a safe and effective method to eliminate the mycoplasma contamination in cultured cells with CIP and BMcyclin.