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目的是探讨标记乳糖基白蛋白的超顺磁性氧化铁粒子（ＬＡＣ－ＨＳＡ－ＳＰＩＯ）作为配体与肝细胞膜去唾液酸糖蛋白受体（ＡＳＧ）特异性结合可能性；评价ＬＡＣ－ＨＳＡ－ＳＰＩＯ增强磁共振受体成像对大鼠微小肝癌灶的显示能力。方法：将Ｆｅ３＋Ｆｅ２＋混合液与ＬＡＣ－ＨＳＡ在碱性条件下制备出ＬＡＯ－ＨＳＡ－ＳＰＩＯ；应用 二乙基亚硝酸胺建立大鼠肝癌模型；电镜及Ｐｅｒｌｓ（普鲁士蓝）染色，观察ＬＡＣ－ＨＳＡ－ＳＰＩＯ在肝组织的颁；并比较ＬＡＣ－ＨＳＡ－ＳＰＩＯ增强前后大鼠肝癌的显示情况。得到结果是（１）Ｐｅｒｌｓ染色证实有ＬＡＣ－ＨＳＡ－ＳＰＩＯ分布到肝细胞浆内；电镜下也显示肝细胞膜表面及胞浆内有电子致密ＳＰＩＯ粒子分布；（２）注射小剂量ＬＡＣ－ＨＳＡ－ＳＰＩＯ可观察到明显明显肝脏强化效果，大鼠肝癌检出率由平扫３６．８％（７／１９）提高到７８％（１５／１９，Ｐ＜００１）。结论：ＬＡＣ－ＨＳＡ－ＳＰＩＯ是一种新型的具有肝细胞ＡＳＧ受体靶向性显影剂，对提高肝脏微小瘤灶检出率有章要价值。
(LAC-HSA-SPIO) as a specific ligand for ligand binding to the asialoglycoprotein receptor (ASG) of the hepatic cell membrane. The purpose of this study was to evaluate the feasibility of using LAC-HSA-SPIO Enhancing MR Imaging of Magnetic Resonance Imaging in Display of Minimal Focal Liver in Rats. METHODS: LAO-HSA-SPIO was prepared under basic conditions with Fe3 + Fe2 + mixed solution and LAC-HSA. Rat hepatocellular carcinoma model was established by diethylnitrosamine. Electron microscopy and Perls (Prussian blue) SPIO in the liver tissue; and compared LAC-HSA-SPIO enhanced liver cancer in rats before and after the show. The results obtained were as follows: (1) LAC-HSA-SPIO was distributed in the cytoplasm of liver cells by Perls staining; electron-dense SPIO particle distribution was observed on the membrane and cytoplasm of liver cells under electron microscope; (2) SPIO can be observed significantly enhanced liver enhancement effect, the detection rate of liver cancer increased from 36.8% (7/19) to 78% (15/19, P <001). CONCLUSION: LAC-HSA-SPIO is a new targeted ASG receptor targeting hepatocyte, which is of value in improving the detection rate of micrometastasis in the liver.