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目的探讨nm23-h1基因对口腔鳞癌细胞增殖的影响及可能机制。方法检测6个口腔鳞癌细胞系中nm23-h1的表达水平;挑选nm23-h1表达量最少、绿色荧光报告基因质粒转染效率最高的细胞系,瞬时转染pCMV-nm23-h1质粒,MTT法检测转染的细胞增殖活性;应用实时定量PCR检测nm23-h1功能相关基因PKC-α的表达。结果在Tca8113、Tb、Tca8113M、TSCC、OSC-4、Tca8113/CDDP6个口腔鳞癌细胞系中,高转移细胞系Tb、Tca8113M的nm23-h1表达量最低。Tb细胞转染pCMV-nm23-h1后,细胞增殖速度减慢,为对照组的79.69%(P<0.01);过表达nm23-h1的Tb细胞PKC-α的mRNA表达量增加,为对照组的1.52倍(P<0.01)。结论nm23-h1基因过表达能抑制口腔鳞癌细胞的增殖,该作用可能与PKC-α相关的信号通路有关。
Objective To investigate the effect of nm23-h1 gene on the proliferation of oral squamous cell carcinoma and its possible mechanism. Methods The expression of nm23-h1 was detected in six oral squamous cell carcinoma cell lines. The cell line transfected with pCMV-nm23-h1 plasmid with the lowest expression of nm23-h1 and green fluorescent reporter plasmids was selected and transfected into pCMV-nm23-h1 plasmid by MTT assay The transfected cell proliferation activity was detected. The expression of nm23-h1 related gene PKC-α was detected by real-time PCR. Results In the Tca8113, Tb, Tca8113M, TSCC, OSC-4, and Tca8113 / CDDP six oral squamous cell carcinoma cell lines, the expression levels of nm23-h1 in the highly metastatic cell lines Tb and Tca8113M were the lowest. After transfected with pCMV-nm23-h1, the cell proliferation rate of Tb cells slowed down to 79.69% of the control group (P <0.01). The mRNA expression of PKC-α in Tb cells over-expressing nm23-h1 increased 1.52 times (P <0.01). Conclusion Overexpression of nm23-h1 can inhibit the proliferation of oral squamous cell carcinoma, which may be related to the signaling pathway associated with PKC-α.