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BALB/C小鼠腹水型肝癌细胞与眼镜蛇毒组分C在无菌条件下混合后接种于昆明种小鼠右前肢腋下,在25℃条件下饲养12天。结果表明,组分C能明显抑制小鼠肝癌细胞的生长(IC50为59.08μg/ml),当肝癌细胞膜被人为造成损伤时,组分C对其生长的抑制作用则显著增强(IC50为6.72μg/ml)。此外,组分C能显著延长荷瘤小鼠的生存时间。对体外培养的小鼠肝癌细胞,组分C也具有很强的杀伤作用,且这种杀伤作用受组分C的浓度、组分C与肝癌细胞孵育的时间及孵育的温度的影响。病理组织检查发现,给组分C后,镜下可见肝癌细胞生长受到明显抑制,肝癌细胞未向皮肤及附件浸润。表明组分C对肝癌细胞生长具有较强的抑制作用
BALB / c mouse ascites hepatocarcinoma cells and cobra venom component C were mixed under sterile conditions and inoculated into the armpit of Kunming mice right anterior forelimb, and kept at 25 ℃ for 12 days. The results showed that component C could significantly inhibit the growth of mouse hepatoma cells (IC50 was 59.08μg / ml). When the cell membrane of human hepatoma was artificially damaged, the inhibitory effect of component C on the growth was significantly enhanced (IC50 6 .72 μg / ml). In addition, component C can significantly prolong the survival time of tumor-bearing mice. For mouse hepatoma cells cultured in vitro, component C also has a strong killing effect, and this killing effect is affected by the concentration of component C, the fraction C and the incubation time of liver cancer cells and incubation temperature. Histopathological examination showed that after the component C, the growth of hepatoma cells was obviously inhibited under the microscope, and the hepatoma cells did not infiltrate the skin and the appendix. Show that component C has a strong inhibitory effect on the growth of hepatoma cells