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The basic principle of optimal method called “moving overlapping resolution mappingMethod” to select the optimal binary mobile phase composition of multi-step linear gradient liquidchromatography is discussed with simultaneously considering effects of position of solute inside thecolumn and mobile phase composition on peak resolution and retention value, then a BASIC programbased on this principle is developed in IBM-PC computer. The validities of both principle ofoptimization and BASIC program are confirmed by separation of samples Containing bile acids andPAHs in RP-HPLC.