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分别于拔节期和收获期取玉米根部及根际土壤样品,采用湿筛-倾注-蔗糖离心法和染色镜检法,测定了AM真菌孢子数量和侵染率;探索分析了不同施肥条件下AM真菌在玉米生长关键时期的变化规律。试验结果表明:拔节期化肥处理的AM真菌孢子数量多于外源几丁质肥料处理;无论是哪种处理,孢子的数量都从拔节期到收获期呈明显下降趋势,而侵染率则呈上升趋势;拔节期孢子数量大于收获期,收获期的菌根侵染率大于拔节期;两种肥料处理均增大孢子侵染率,但外源几丁质肥料的增大幅度明显高于化学肥料处理,增大13.91倍,几丁质肥料处理孢子的减少率与菌根侵染提高率呈正相关性;本试验条件下,认为通过不同的施肥方式,AM真菌对玉米作物的菌根侵染、产孢数量具有一定的影响和规律性,几丁质肥料能够创造更加适宜的菌根侵染条件,为AM真菌规律变化的探索研究提供理论依据。
The AM fungi spore number and infection rate were determined by wet sieving-pouring-sucrose centrifugation and staining microscopy at the jointing and harvesting stages respectively. The effects of AM Changes of fungi in the critical period of corn growth. The results showed that the number of spores of AM fungi treated by jointing fertilizer was more than that of exogenous chitin fertilizer. No matter what kind of treatment, the number of spores decreased obviously from jointing stage to harvesting stage while the infection rate was The number of spores at the jointing stage was larger than the harvesting stage, and the mycorrhizal infection rate at the harvest stage was greater than that at the jointing stage. Both treatments increased the spore infection rate, but the increase of the exogenous chitin fertilizer was obviously higher than that of the chemical Fertilizer treatment, an increase of 13.91 times, the reduction rate of chitin fertilizer treatment spores and mycorrhizal infection rate was positively correlated; under the conditions of this experiment, that different AMF fungal infection of corn plants , The number of sporulation has a certain influence and regularity. Chitin fertilizer can create more suitable conditions for mycorrhizal infection, and provide a theoretical basis for the exploration of the regular changes of AM fungi.