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茴香酸对羟基苯乙酯(HPA)是中药羌活和宽叶羌活的主要生物活性成分之一.本文建立了一种快速、特异的反相高效液相色谱法(RP-HPLC)用于静脉(20 mg/kg)和灌胃(200 mg/kg)给予大鼠HPA后其在大鼠血浆的测定和药代动力学研究.血浆样品经乙酸乙酯液液萃取净化后,进样RP-HPLC系统分离和测定.分析型色谱柱为DiamonsilTMODS C18;血浆样品分析的流动相为MeOH-H2O(v/v),静脉给药样品分析时为75∶25,灌胃给药样品分析时为70∶30;流速为1.0 mL/min,UV检测波长为256 nm.HPA标准曲线线性范围,静脉给药时为0.05-5.0 μg/mL(r2=0.9984),灌胃给药时为0.5-10.0 μg/mL(r2 = 0.9995);萃取回收率为82.01％-87.97％,日内和日间精密度为1.71％-3.99％,准确度范围为91.22％-110.5％;HPA在大鼠口服时的绝对生物利用度为48.17％.以上结果表明所建方法适合用于HPA在大鼠血浆中的测定和药代动力学研究.“,”In the present study,we developed a rapid and specific reversed-phase high-performance liquid chromatographic(RP-HPLC)method for the quantification ofp-hydroxyphenethyl anisate(HPA),one of the main bioactive constituents of the roots and rhizomes of Notopterygium incisum and N.franchetii,in rat plasma after an intravenous(20 mg/kg,i.v.)and an intragastrical(200 mg/kg,i.g.)administration to rats,respectively.The method involved a plasma clear-up step using liquid-liquid extraction by EtOAc,followed by RP-HPLC separation and detection.Separation of HPA was performed on an analytical DiamonsilTM ODS C18 column with the mobile phase of MeOH-H2O at ratios of 75∶25(v/v)for i.v.and 70∶30(v/v)for i.g.administration.The flow-rate was 1.0 mL/min,and UV detection was performed at 256 nm.The calibration curves were linear over the ranges of 0.05-5.0 pg/mL(r2 = 0.9984)for i.v.and 0.5-10.0 pg/mL(r2= 0.9995)for i.g.administration in rat plasma.The extraction recoveries were in the range of 82.01％-87.97％.The intra-and inter-day precisions were between 1.71％and 3.99％,with accuracies ranging from 91.22％to 110.5％.The absolute bioavailability of an orally administered HPA in rats was about 48.17％.The developed method was suitable for the determination and nharmacokinetic study of HPA in rat nlasma.