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目的:研究胃癌中DNA倍体及其TIMP-2和E-cadherin的表达,探索胃癌侵袭转移的分子基础和可能机制。方法:采用免疫组化技术检测E-cadherin、TIMP-2在99例胃癌,16例癌周正常黏膜,16例胃癌远处转移和25例胃癌转移阳性的淋巴结中表达情况;选取其中47例胃癌,6例癌周正常黏膜及4例胃癌远处转移标本采用流式细胞术检测DNA倍体及S期分数。结果:TIMP-2表达与Borrmann’s分型、淋巴结转移和浸润深度有关;E-cadherin表达与肿瘤细胞分化、Lauren’s分型、Borrmann’s分型、淋巴结转移和浸润深度有关。DNA异倍体率与分化和淋巴结转移有关,S期分数(SPF)与肿瘤大小、分化及淋巴结转移有关。而且在癌与癌周非癌黏膜之间E-cadherin表达、DNA异倍体率和S期分数的差别具有统计学意义;TIMP-2与E-cadherin之间无相关性;E-cadherin表达与DNA倍体及S期分数呈正相关。结论:随着肿瘤的演进和异质化,TIMP-2和E-cadherin的异常表达及DNA异倍体和高S期分数也相应逐渐增加,提示它们在胃癌演进过程中起着关键作用,可以作为胃癌生物学行为的客观标志物。而且,这几种因素间的相互作用更加速了肿瘤演进过程。
Objective: To study the expression of DNA ploidy, TIMP-2 and E-cadherin in gastric cancer and to explore the molecular basis and possible mechanism of gastric cancer invasion and metastasis. Methods: The expressions of E-cadherin and TIMP-2 in 99 gastric cancer, 16 normal mucosa, 16 distant metastasis of gastric cancer and 25 lymph node metastasis of gastric cancer were detected by immunohistochemistry. 47 cases of gastric cancer , 6 cases of normal mucosa and 4 cases of distant metastasis of gastric cancer using flow cytometry DNA ploidy and S phase score. Results: The expression of TIMP-2 was related to Borrmann’s classification, lymph node metastasis and depth of invasion. E-cadherin expression was correlated with tumor cell differentiation, Lauren’s classification, Borrmann’s classification, lymph node metastasis and depth of invasion. DNA aneuploidy rate and differentiation and lymph node metastasis, S phase score (SPF) and tumor size, differentiation and lymph node metastasis. The difference of E-cadherin, DNA aneuploidy and S-score between cancer and non-cancerous mucosa was statistically significant. There was no correlation between TIMP-2 and E-cadherin. The expression of E-cadherin DNA ploidy and S-phase score was positively correlated. CONCLUSIONS: With the evolution and heterogeneity of tumors, the abnormal expression of TIMP-2 and E-cadherin and the corresponding increase of DNA aneuploidy and high S phase suggest that they play a key role in the evolution of gastric cancer, As an objective marker of biological behavior of gastric cancer. Moreover, the interaction between these factors speeds up the evolution of the tumor.