论文部分内容阅读
目的:建立同时测定十味板蓝根颗粒中尿苷、腺苷、表告依春、靛玉红含量的方法。方法:采用超高效液相串联质谱法(UPLC-MS/MS)。色谱柱为Agilent ZORBAX SB-C_(18),流动相为甲醇-0.1%甲酸溶液(等度洗脱),采用电喷雾电离源,多反应离子监测(MRM),用于定量分析的尿苷、腺苷、表告依春、靛玉红检测离子对m/z分别为243.0/111.0、268.1/136.0、127.9/58.0、263.1/219.0。外标法定量分析。结果:尿苷、腺苷、表告依春、靛玉红的质量浓度范围分别在10~320、7.5~240、2.5~80、7.5~240 ng·m L~(-1)范围内与各自的峰面积呈良好的线性关系(r=0.9924、0.9943、0.9965、0.9987)。加样回收率在91.3%~105.3%范围内,RSD%均低于4.2%。结论:该法专属性强、快速灵敏,可用于十味板蓝根颗粒中尿苷、腺苷、表告依春、靛玉红的含量测定。
Objective: To establish a method for simultaneous determination of uridine and adenosine in ten-flavonized Banlangen granules according to the content of indirubin and indirubin. Methods: Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) was used. The column was Agilent ZORBAX SB-C 18 with a mobile phase of methanol-0.1% formic acid (isocratic elution) using an electrospray ionization source, multiple reaction ion monitoring (MRM), uridine for quantitative analysis, Adenosine, the table according to spring, indirubin ion pairs detected m / z 243.0 / 111.0,268.1 / 136.0,127.9 / 58.0,263.1 / 219.0. External standard method of quantitative analysis. Results: The concentrations of uridine, adenosine and epirubicin in the range of 10-320, 7.5-240, 2.5-80 and 7.5-240 ng · m L -1 respectively The peak area showed a good linear relationship (r = 0.9924,0.9943,0.9965,0.9987). The recoveries of samples ranged from 91.3% to 105.3% with RSD% below 4.2%. Conclusion: The method is highly specific, fast and sensitive, and can be used for the determination of uridine and adenosine in the ten-flavonized Banlangen granules.