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分别收集恩施（高硒地区）、北京（适硒地区）、启东（肝癌高发区）、天水和延边（低硒大骨节病区）不同硒水平地区的大豆样品，研究硒形态和分布的自然情况及其地区差异。大豆蛋白组分的分离采用Ｔｈａｎｈ和Ｓｈｉｂａｓａｋｉ报道的方法，并用ＤＡＮ－荧光检测法分别测定大豆粉、脱脂大豆粉、固体残渣、１１Ｓ蛋白、７Ｓ蛋白、乳清蛋白、液体残留物中的硒含量。结果表明：（１）４２．６％～６２．６％的硒结合于水溶性蛋白上，硒的含量水平（０．１００～２．３３８ｍｇＳｅ／ｋｇ）不因地区而异。蛋白组分对硒有明显的富集作用，富集硒的倍数为１．２～２．３；（２）硒在各蛋白组分间的绝对含量分布有地区间差异和组分问差异，各组分对硒的富集倍数有以下顺序：恩施地区；１１Ｓ蛋白＞７Ｓ蛋白）乳清蛋白，其它地区：乳清蛋白＞１１Ｓ蛋白＞７Ｓ蛋白。在１１Ｓ蛋白、７Ｓ蛋白组分中的相对百分分布地区问差异小，在乳清蛋白中的百分分布地区间差异则较大；（３）液体残留物（主要为小分子物质）结合硒的能力有限，在不同硒含量样品中，晒绝对含量不发生显著性的变化。
Soybean samples from different selenium levels in Enshi (high selenium area), Beijing (suitable selenium area), Qidong (high incidence area of liver cancer), Tianshui and Yanbian (low selenium Kashin-Beck disease area) were collected to study the natural situation of selenium morphology and distribution And their regional differences. Soybean protein fractions were separated by the method reported by Thanh and Shibasaki and the content of selenium in soybean meal, defatted soybean meal, solid residue, 11S protein, 7S protein, whey protein and liquid residue was determined by DAN-fluorescence detection. The results showed that: (1) 42.6% -62.6% selenium bound to water-soluble protein, selenium level (0.100-2.338mgSe / kg) did not vary by region. The protein component enriched the selenium significantly, and the multiple of selenium was 1.2 ~ 2.3. (2) The absolute content distribution of selenium in each protein component ranged from region to region and the difference of components was different. Whey protein, whey protein, 11S protein> 7S protein. Other regions: Whey protein> 11S protein> 7S protein. In the 11S protein, 7S protein components in the relative percentage distribution of regional asked the difference is small, the percentage difference in the whey protein range between the larger; (3) liquid residues (mainly small molecules) combined with selenium Of the limited capacity, in different selenium content samples, absolute content of the sun did not change significantly.