论文部分内容阅读
从四川攀枝花市田间采集36份表现严重矮化、黄化和曲叶症状的番茄病株样本,利用双生病毒简并引物PA/PB从所有样本中均扩增得到约500 bp的片段,经全序列测定及分析,检测出中国番木瓜曲叶病毒(Papaya leaf curl China virus,PaLCuCNV)和中国番茄黄化曲叶病毒(Tomato yellow leaf curl China virus,TYLCCNV),这两种双生病毒的复合侵染率达97.2%。系统进化分析表明,这两种双生病毒分别与已报道的PaLCuCNV河南番茄分离物(PaLCuCNV-[HeNZMI])及TYLCCNV云南元谋烟草分离物(TYLCCNV-[Y295])的核苷酸序列相似性最高,分别为99.1%和97.9%。检测发现,所有分离物均伴随有卫星DNAβ分子,全序列测定表明所得9个DNAβ分子均为TYLCCNV的卫星TYLCCNB,且与其四川番茄分离物(TYLCCNB-[SC65])的核苷酸序列相似性最高,为87.7%~94.5%。本文首次报道PaLCuCNV与TYLCCNV/TYLCCNB病害复合体复合侵染番茄引起更严重的番茄黄化曲叶病。
A total of 36 tomato diseased plants with severe dwarfing, yellowing and leaf curling symptoms were collected from Panzhihua, Sichuan Province. Approximately 500 bp fragments were amplified from all the samples using twin virus degenerate primers PA / PB. Sequence analysis and sequencing showed that the two Chinese isolates of Papaya leaf virus (PaLCuCNV) and Tomato yellow leaf curl China virus (TYLCCNV) Rate of 97.2%. Phylogenetic analysis showed that the two geminiviruses had the highest similarity with the reported PaLCuCNV Henan tomato isolate (PaLCuCNV- [HeNZMI]) and the TYLCCNV Yunnan Yuanmou tobacco isolate (TYLCCNV- [Y295]), respectively. Respectively 99.1% and 97.9%. The results showed that all the isolates were accompanied by DNAβ molecules, and the complete sequence analysis showed that all the nine DNAβ molecules were TYLCCNB of TYLCCNV, and had the highest similarity with the Sichuan tomato isolate (TYLCCNB- [SC65]) , 87.7% ~ 94.5%. This paper reports for the first time that the combination of PaLCuCNV with the TYLCCNV / TYLCCNB disease complex causes a more severe tomato yellow leaf curl disease in tomatoes.