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建立HPLC紫外检测器的方法来检测安神补脑液中维生素B1的含量,用以改进现行标准中的方法。采用安捷伦SB-C18柱,以0.02 mol/L磷酸二氢铵-0.025 mol/L庚烷磺酸钠溶液(磷酸pH调至3.0)∶乙腈(80∶20)为流动相,检测波长为245 nm。线性范围为2.08~41.6μg/mL,相关系数R2=1.000,线性关系良好。该方法专属性好,准确、灵敏,解决了现行方法出现的问题,并简化了前处理步骤,更适合于VB1的含量测定。
The method of HPLC UV detector was established to detect the content of vitamin B1 in Anshen Bunao Solution to improve the current standard methods. An Agilent SB-C18 column was used with a mobile phase of 0.02 mol / L ammonium phosphate-0.025 mol / L sodium heptanesulfonate solution (phosphoric acid pH adjusted to 3.0): acetonitrile (80:20) . The linear range of 2.08 ~ 41.6μg / mL, correlation coefficient R2 = 1.000, good linear relationship. The method has the advantages of good specificity, accuracy and sensitivity, solves the problems existing in the existing methods, and simplifies the pre-treatment steps and is more suitable for the determination of the content of VB1.