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1982年在不列颠哥伦比亚省的Kamloops林区设置4块10ha受黄杉毒蛾(Orgyia pseudotsugata)为害的花旗松林样地。当大部分幼虫处于1龄期时,应用飞机对样地喷洒核型多角体病毒(Virtuss)。其中一块林地喷洒乳油病毒混合液,使用剂量为2.5×10~(11)PIB/ha(PIB为多角体包涵体);另一块样地喷洒含糖蜜的病毒水混合液,使用剂量与前者相同;其他两块样地喷洒乳油病毒混合液,使用剂量分别为8.3×10~(10)PIB/ha及1.6×10~(10)PIB/ha。用定翼飞机进行喷洒,喷咀口径定为每公顷喷洒9.4L病毒混合液。另选4块样地作为空白对照。通过Abbott校正公式计算,飞机喷洒病毒6周后,使用最低病毒剂量样地的虫口降低65％,而其余3块样地的虫口降低87～95％。飞喷病毒5～6周后,通过镜检,幼虫的病毒感染率高达85～100％,对照样地幼虫的病毒感染率很低,从病毒处理样地采回的虫茧羽化率为4～19％,而对照样地为28～43％。病毒处理样地的卵块密度明显降低,其中一块降低97％,有二块降低90％,另一块没有测定。 8.3×10~(10)PIB/ha的使用剂量(仅是推荐剂量的1/3)就足够了,与乳油混合使用也是可行的。
In 1982, four 10-ha-shaped samples of Douglas fir forest contaminated with Orgyia pseudotsugata were set up in the Kamloops forest area of British Columbia. When most of the larvae are in the first instar, the aircraft is sprayed with the sample of nuclear polyhedrosis virus (Virtuss). One piece of woodland was sprayed with a mixture of ECV virus at a dose of 2.5 × 10-11 PIB / ha (PIB was a polyhedron inclusion body); the other sample was sprayed with a mixture of water and molasses with molasses at the same dosage as the former; The other two samples were sprayed with the mixture of ECV at doses of 8.3 × 10 ~ (10) PIB / ha and 1.6 × 10 ~ (10) PIB / ha, respectively. Spray with fixed-wing aircraft, nozzle diameter set at 9.4L per hectare virus mixture. Another 4 plots as a blank control. Abbott’s calibration formula calculated that after 6 weeks of spraying the virus, the population with the lowest virus dose reduced by 65% and the remaining 3 samples decreased by 87% -95%. The virus infection rate of larvae reached as high as 85-100% after 5 to 6 weeks of flying spray virus. The virus infection rate of the control sample larvae was very low, and the emergence rate of the insect cocoon recovered from the virus-treated land samples was 4 ~ 19%, while the control sample is 28 ~ 43%. The egg mass density in the treated area was significantly reduced, with 97% reduction in one, 90% reduction in two, and no determination in the other. 8.3 × 10 ~ (10) PIB / ha use of the dose (only the recommended dose of 1/3) is sufficient, and cream oil is also feasible.