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目的建立肾康分散片干膏中黄芪甲苷含量的高效液相色谱法测定方法。方法以黄芪甲苷的含量为指标,考察液相色谱法色谱条件、流动相、线性范围、样品制备方法。结果流动相为乙腈-水(32:68)洗脱,线性范围:17.9-178.5 ug/m L,黄芪甲苷的平均含量为0.1359 mg/g,RSD=2.21%;回收率为98.16%,RSD为2.65%。结论本方法操作简便,精确度高,可靠性强,可作为肾康分散片干膏中黄芪甲苷的含量测定方法。
Objective To establish a HPLC method for the determination of Astragaloside IV in Shenkang Dispersible Tablets. Methods The content of astragaloside Ⅳ was taken as an index to investigate the chromatographic conditions, mobile phase, linear range and sample preparation methods of liquid chromatography. Results The mobile phase was eluted with acetonitrile-water (32:68). The linear range was 17.9-178.5 ug / m L. The average content of astragaloside was 0.1359 mg / g, RSD was 2.21%. The recovery was 98.16% 2.65%. Conclusion This method is simple, accurate, and reliable, and can be used as a method for the determination of Astragaloside IV in Shenkang dispersible tablets.