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目的探讨爪蟾抗菌肽(magainin)的人工合成及对金黄色葡萄球菌细胞膜的作用。方法利用氨基酸固相合成的方法合成爪蟾抗菌肽,高效液相色谱法进行纯化,质谱鉴定后,得到的爪蟾抗菌肽纯度在99%以上。将爪蟾抗菌肽作用于引起感染性心内膜炎的金黄色葡萄球菌ATCC25923,以PBS缓冲液作为阴性对照,TritonX-100作为阳性对照。结果爪蟾抗菌肽作用于引起心内膜炎的金黄色葡萄球菌后,ATCC25923的630 nm值从0.166下降到0.138,负染后透射电镜观察,发现细菌表面存在破损成小孔的地方,细胞膜界限模糊不清,甚至细胞膜完全溶解。爪蟾抗菌肽通过破坏细菌细胞膜而导致细菌的死亡。结论爪蟾抗菌肽能够提高细菌细胞膜的通透性。
Objective To investigate the artificial synthesis of magainin and its effect on the cell membrane of Staphylococcus aureus. Methods The antibacterial peptides of magainin were synthesized by solid phase synthesis of amino acids and purified by high performance liquid chromatography (HPLC). The purity of the antibacterial peptides was more than 99%. Xenopus antimicrobial peptides were administered to S. aureus ATCC25923 causing infective endocarditis, PBS buffer as a negative control and Triton X-100 as a positive control. Results After Xenopus antimicrobial peptides were applied to cause endocarditis in Staphylococcus aureus, the value of 630 nm of ATCC25923 decreased from 0.166 to 0.138. After negative staining, transmission electron microscopy showed that the surface of bacteria was broken into small holes, Limits blurred, even the cell membrane completely dissolved. Xenopus antimicrobial peptides cause bacterial death by disrupting bacterial cell membranes. Conclusion Xenopus antimicrobial peptides can improve the permeability of bacterial cell membrane.