菌种生产一般分为三级,即母种、原种和栽培种.母种,即试管菌种,是由孢子或菇体组织分离所得的最初菌种;将母种扩大到棉籽壳或其他培养基上培育的菌丝称为原种;原种再扩大培养一次即为栽培种.栽培种培养基的配方及培养方法与原种相同.一、制种仪器及工具 The production of bacteria is generally divided into three levels, that is, the mother species, the original species and cultivars. The mother species, that is, test tube strains, is the initial species isolated from spores or mushroom body tissue; the mother expanded to cottonseed hulls or other The mycelium grown on the medium is called the original species; the original species is expanded and cultivated once, which is the cultivated species. The formula and cultivation method of the cultivated species are the same as the original species.