论文部分内容阅读
目的:对仪器法与显微镜目测法(手工法)计数网织红细胞进行比较,对手工法和仪器法测定网织红细胞进行方法学评价。方法:采用全自动血液分析仪法和煌焦油蓝手工法分别计数网织红细胞。结果:用仪器法与手工法测得的网织红细胞结果经过统计学分析,P>0.05,两种方法所得结果无显著性差异,但仪器法测定结果的平均变异系数经计算为高值1.34%、中值3.30%、低值2.67%,而手工法为高值6.66%、中值14.0%、低值21.6%,手工法平均变异系数明显高于仪器法。当分组对照时仪器法与手工法测得的网织红细胞结果经过统计学分析,P<0.05,两种方法所得结果有显著性差异。结论:仪器法与手工法测定网织红细胞结果相关性好,但仪器法精密度高,重复性好,省时省力,适合大批量标本的快速检测,而手工法观察网织红细胞形态比较直观,可用于对仪器法检测的异常结果的复核。
OBJECTIVE: To compare the reticulocyte counts between the instrumental method and the microscope (manual method), and to evaluate the methodological reworking of the reticulocytes by manual method and instrumental method. Methods: The reticulocytes were counted by automatic hematology analyzer and brilliant tar blue manual method respectively. Results: The reticulocyte results obtained by instrumental method and manual method were statistically analyzed (P> 0.05). The results obtained by the two methods showed no significant difference. However, the average coefficient of variation of the instrumental method was 1.34% , Median 3.30%, low 2.67%, while the manual method was 6.66% high, median 14.0%, low 21.6%, the average manual method was significantly higher than the instrumental method. When the control group and manual method of measuring the reticulocyte results after statistical analysis, P <0.05, the results obtained by the two methods were significantly different. Conclusion: The instrumental method and manual method have good correlation with the results of reticulocyte. However, the instrumental method has the advantages of high precision, good repeatability, time-saving and labor-saving, suitable for the rapid detection of large quantities of specimens. However, Can be used to check the abnormal results of instrumentation.