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目的：探讨肝素是否能抑制生长因子诱导的大鼠肺动脉平滑肌细胞（ＰＡＳＭＣ）分裂和增殖．方法：应用含１０％ＦＢＳ的Ｍ１９９培养液培养大鼠ＰＡＳＭＣ．细胞分裂及细胞增殖分别用［ｍｅｔｈｙｌ３Ｈ］ＴｄＲ和细胞计数监测．结果：ＦＢＳ（１０％），以及ＦＢＳ（１％）与ＰＤＧＦ（５０μｇ·Ｌ－１），ＦＧＦ（５０μｇ·Ｌ－１），或ＩＬ１α（１００ｎｇ·Ｌ－１）联合应用均能增加大鼠ＰＡＳＭＣ分裂．肝素（１００ｍｇ·Ｌ－１）抑制１０％ＦＢＳ诱导的大鼠ＰＡＳＭＣ增殖（２８％±６％）和胸腺嘧啶摄取反应（２７％±７％），抑制ＦＢＳ（１％）与ＰＤＧＦ（５０μｇ·Ｌ－１），ＦＧＦ（５０μｇ·Ｌ－１），或ＩＬ１α（１００ｎｇ·Ｌ－１）联用诱导的大鼠ＰＡＳＭＣ增殖（２５％±６％，２７％±７％，２０％±４％），以及胸腺嘧啶摄取反应（２３％±７％，２６％±６％，２０％±６％）．结论：肝素抑制生长因子诱导的大鼠ＰＡＳＭＣ的分裂与增殖．
Objective: To investigate whether heparin can inhibit growth factor induced pulmonary artery smooth muscle cells (PASMC) division and proliferation. Methods: Rat PASMC was cultured in M199 medium containing 10% FBS. Cell division and cell proliferation were monitored with [methyl-3H] TdR and cell count, respectively. Results: The combination of FBS (10%), FBS (1%) and PDGF (50μg · L-1), FGF (50μg · L-1) or IL1α (100ng · L-1) Rat PASMC division. Heparin (100 mg · L-1) inhibited 10% FBS-induced rat PASMC proliferation (28% ± 6%) and thymidine uptake (27% ± 7% (25% ± 6%, 27% ± 7%, 20% ± 4%) of PASMC induced by FGF (50μg · L-1) or IL1α (100ng · L- ), And thymine uptake responses (23% ± 7%, 26% ± 6%, 20% ± 6%). Conclusion: Heparin inhibits the growth and differentiation of rat PASMC induced by growth factor.