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PCR技术被认为是当今分子生物学发展最重要的生物学技术之一。原位PCR技术结合了具有细胞定位能力的原位杂交技术及具有高度敏感和特异的PCR技术,成为一项在组织细胞学方面的临床诊断及科学研究中具有极大潜力的新型技术。本文就组织细胞内的原位PCR技术及应用作一简述。
PCR technology is considered as one of the most important biological techniques in the development of molecular biology. In situ PCR combines in situ hybridization with cell localization capabilities with highly sensitive and specific PCR techniques to become a new technology with tremendous potential for clinical diagnosis and scientific research on histiocytology. In this paper, in situ cell-based in situ PCR techniques and applications for a brief review.