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利用SRAP分子标记技术构建了22份高丹草品种的指纹图谱。该指纹图谱可以准确区分供试的所有22个高丹草品种,置信概率达到99.9 999%,为高丹草品种划分提供了基础。利用筛选出的19个多态性较好的SRAP引物组合对22个高丹草品种进行遗传多样性分析鉴定,共扩增出450个位点,其中多态性位点369个;平均每个引物组合产生23.6个位点和19.4个多态性位点,平均多态性水平为82.2%。通过采用UPGMA方法进行聚类分析,遗传相似系数在0.66～0.94之间,以遗传相似系数0.674为阈值,可将供试材料分为2个类群。
Fingerprints of 22 cultivars of Sorghum bicolor were constructed using SRAP molecular markers. The fingerprinting can accurately distinguish all of the 22 cultivars tested, with a confidence probability of 99.9999%, which provides the basis for the differentiation of cultivars. Twenty-nine cultivars of Sorghum bicolorwere analyzed by genetic diversity using 19 selected SRAP primer combinations. A total of 450 loci were amplified, of which 369 were polymorphic loci. On average, The primer combination produced 23.6 loci and 19.4 polymorphic loci with an average of 82.2%. By using UPGMA clustering analysis, the genetic similarity coefficients were between 0.66 and 0.94, and the genetic similarity coefficient 0.674 was the threshold value, which could be divided into two groups.