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目的探讨检测胃癌患者外周血中K-ras和p53基因突变的临床意义。方法应用聚合酶链反应-单链构象多态性分析(PCR-SSCP)法,对我院收治的73例胃癌患者外周血中的K-ras和p53基因突变进行检测,并与20例非肿瘤疾病患者作对照。结果73例胃癌患者中有7例外周血中检测出p53基因突变,阳性率为9.6%;5例检测出K-ras基因突变,阳性率为6.8%。对照组外周血中均未检出K-ras和p53基因突变。有无肝转移的胃癌患者外周血p53和K-ras基因突变阳性率间差异均有统计学意义(P<0.05),不同分化程度及临床分期的胃癌患者外周血p53和K-ras基因突变阳性率间差异无统计学意义(P>0.05)。结论PCR-SSCP法检测外周血中K-ras和p53基因DNA有助于胃癌肝转移的诊断。
Objective To investigate the clinical significance of detecting K-ras and p53 gene mutations in peripheral blood of patients with gastric cancer. Methods The mutations of K-ras and p53 in peripheral blood of 73 patients with gastric cancer admitted to our hospital were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and compared with 20 non-tumor Disease patients as a control. Results The p53 gene mutation was detected in 7 out of 73 cases of gastric cancer. The positive rate of p53 gene was 9.6%. The mutation rate of K-ras gene in 5 cases was 6.8%. No K-ras and p53 gene mutations were detected in peripheral blood of the control group. The positive rates of p53 and K-ras gene mutations in peripheral blood of patients with and without liver metastasis were significantly different (P <0.05). The positive rates of p53 and K-ras gene mutations in peripheral blood of patients with different degrees of differentiation and clinical stage were positive No significant difference between rates (P> 0.05). Conclusion The detection of K-ras and p53 gene DNA in peripheral blood by PCR-SSCP is helpful for the diagnosis of hepatic metastasis of gastric cancer.