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目的:建立高效液相色谱法同时测定肝复康丸中五味子醇甲、五味子酯甲、五味子甲素和五味子乙素等4种成分的含量测定方法。方法:采用Agilent TC-C_(18)色谱柱(250 mm×4.6 mm,5μm);乙腈-水为流动相,梯度洗脱,流速0.8 ml·min~(-1);检测波长:254 nm。结果:五味子醇甲、五味子酯甲、五味子甲素、五味子乙素与其相邻杂质峰能完全分离,五味子醇甲、五味子酯甲、五味子甲素、五味子乙素的线性范围(相关系数)分别为68.62～686.25μg·ml~(-1)(r=0.999 9),15.54～155.40μg·ml~(-1)(r=0.999 8),6.45～64.50μg·ml~(-1)(r=0.999 9)和15.57～155.70μg·ml~(-1)(r=0.999 9)。平均回收率分别为100.80%,100.46%,101.06%和100.55%,RSD为1.31%,1.72%,2.00%和1.67%。结论:本方法简便、准确、重复性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of four components of schisandra chinensis, schisandrin, schisandrin and schisandrin B in. Methods: Agilent TC-C 18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and water with gradient elution at a flow rate of 0.8 ml · min -1. The detection wavelength was 254 nm. Results: Schisandra alcohol A, Schisandra chinensis A, Schisandrin B and Schisandrin B were completely separated from the adjacent impurity peaks. The linear range (correlation coefficient) of schisandrin A, Schisandra A, Schisandrin B and Schisandrin B were 68.62 ~ 686.25μg · ml -1 (r = 0.999 9), 15.54 ~ 155.40μg · ml -1 (r = 0.999 8), 6.45 ~ 64.50μg · ml -1 (r = 0.999 9) and 15.57-155.70 μg · ml -1 (r = 0.999 9). The average recoveries were 100.80%, 100.46%, 101.06% and 100.55%, respectively, with RSDs of 1.31%, 1.72%, 2.00% and 1.67%. Conclusion: The method is simple, accurate, reproducible and can eliminate the interference of other components and can be used for the quality control evaluation of the preparation.