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目的分析河南驻马店市经性传播的艾滋病病毒(HIV)感染者中,HIV流行毒株亚型及其毒株特征。方法通过专业问卷调查,了解驻马店地区经性传播HIV感染者的基本状况。采集感染者的EDTA抗凝静脉血,检测其CD+4T淋巴细胞(简称CD4细胞)计数,提取外周血浆中病毒核糖核酸(RNA),使用反转录巢式荧光定量聚合酶链反应(PCR)方法扩增病毒全长gag和部分pol基因,利用Clustal软件比对序列,利用BioEdit软件手工校对,利用NCBI网站HIV分型工具分析病毒亚型,利用MEGA软件构建NJ树,分析病毒系统进化关系。结果共收集到HIV感染者血液74份,全部经性传播感染HIV。经过测序,共获得HIV全长gag基因56条(75.7%),全长pol基因38条(51.4%)。对61例HIV感染者感染病毒亚型进行了分析,其中B亚型毒株47例(77.0%),CRF01_AE毒株6例(9.8%),CRF07_BC毒株4例(6.6%),C亚型毒株2例(3.3%),新型重组毒株2例(3.3%)。系统进化分析显示,所有的B亚型毒株均为Thai B毒株。38例获得pol区序列的样本中,有4例具有耐药突变。结论驻马店市性传播HIV感染者中流行的HIV毒株构成复杂,Thai B亚型毒株是优势毒株,CRF01_AE、CRF07_BC、C等亚型毒株均已进入该人群,而且出现了新型重组毒株。针对该人群的HIV流行毒株监测至关重要。
Objective To analyze the subtypes of HIV epidemic strains and their virus strains among sexually transmitted HIV patients in Zhumadian City, Henan Province. Methods Through professional questionnaire survey, understand the basic status of sexually transmitted HIV infection in Zhumadian area. Collect the anticoagulated venous blood of infected persons and detect the counts of CD + 4T lymphocytes (CD4 cells for short), extract the peripheral blood plasma RNA (RNA), use reverse transcription nested fluorescent quantitative polymerase chain reaction (PCR) Methods The full-length gag and partial pol genes of the virus were amplified. The sequences were aligned by Clustal software and manually calibrated by BioEdit software. The virus subtypes were analyzed by NCBI website and the NJ tree was constructed by using MEGA software to analyze the evolutionary relationship of the virus system. Results A total of 74 HIV-infected patients were collected, all of whom were sexually transmitted through HIV. After sequencing, 56 full-length gag genes (75.7%) and 38 full-length pol genes (51.4%) were obtained. The subtypes of 61 HIV-infected individuals were analyzed, of which 47 (77.0%) were B subtype, 6 (9.8%) were CRF01_AE, 4 (6.6%) were CRF07_BC, Two strains (3.3%) and two new strains (3.3%) were isolated. Phylogenetic analysis showed that all B subtype strains were Thai B strains. Of the 38 samples that obtained the pol region, 4 had resistance mutations. Conclusion The prevalence of HIV strains among sexually transmitted HIV-infected persons in Zhumadian City is complex. The Thai B subtype strains are the predominant strains. The subtypes CRF01_AE, CRF07_BC and C have entered this population, and new recombinant viruses have emerged Strain. Monitoring of HIV-infected strains of this population is crucial.