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中药生物药剂学分类系统(biopharmaceutics classification system of Chinese materia medica,CMMBCS)研究中,多成分同时测定时需要考虑成分间吸收过程中的相互作用。该研究以荷叶为研究载体,采用离体翻转肠囊实验方法,对荷叶中的多成分在肠道的吸收情况进行研究,并对已知成分进行定量分析。实验表明荷叶提取物中有19个成分可透过肠壁吸收,各成分吸收强弱差异比较大,3个浓度下已知成分荷叶碱的表观渗透系数在1×10-5~1×10-6cm·s-1数量级,其他已知成分的表观渗透系数数量级分别为芦丁1×10-6~1×10-7cm·s-1,金丝桃苷1×10-6cm·s-1,异槲皮苷1×10-6~1×10-7cm·s-1,紫云英苷1×10-6~1×10-7cm·s-1,在多成分吸收影响下,几个成分均属于肠渗透性差的成分。这为下一步对不同类别成分分别进行相关靶点研究奠定基础。
In the study of biopharmaceutics classification system of Chinese materia medica (CMMBCS), the simultaneous determination of multiple components needs to consider the interaction between the components in the absorption process. In this study, the lotus leaf was used as the research carrier, and the intestine absorption of multiple components in lotus leaf was studied by using the method of turning over the intestine in vitro, and the quantitative analysis of the known components was carried out. Experiments show that there are 19 components in lotus leaf extract can be absorbed through the intestinal wall, the absorption intensity of each component is relatively large, three concentrations of known ingredients lotus leaf base of the apparent permeability coefficient of 1 × 10-5 ~ 1 × 10-6cm · s-1, the apparent permeability coefficient of other known components are respectively rutin 1 × 10-6 ~ 1 × 10-7cm · s-1, hyperin 1 × 10-6cm · s-1, isoquercitrin 1 × 10-6 to 1 × 10-7cm · s-1, and orientin 1 × 10-6 to 1 × 10-7cm · s-1. Under the influence of multi-component absorption , Several ingredients are part of intestinal permeability of the poor ingredients. This will lay the foundation for the next step to carry out relevant target research separately for different categories of ingredients.