目的:研究小鼠切牙颈环上皮细胞的培养方法。方法:提取出生后7～8 d的小鼠下颌切牙唇侧根尖端组织,采用酶消化组织块法进行细胞培养。用角蛋白免疫化学染色对细胞来源进行鉴定。结果:组织块酶消化后接种于培养板,3～7 d左右可见细胞爬出,原代培养细胞为成纤维细胞及上皮细胞混杂,胰酶差别消化后,获得纯化的上皮细胞。角蛋白染色阳性,证明其为上皮来源细胞。结论:采用酶消化组织块法成功培养小鼠切牙颈环上皮细胞。 Objective: To study the culture method of the incisor cervical collar epithelial cells in mice. Methods: The labial apical tissues of mandibular incisors were extracted from 7 to 8 days after birth, and the cells were cultured by enzyme digestion. Cell origin was identified by keratin immunochemical staining. RESULTS: Tissue samples were inoculated into culture plates after enzymatic digestion, and cells were seen to climb out within about 3 to 7 days. Primary cultured cells were mixed with fibroblasts and epithelial cells, and purified epithelial cells were obtained after differential digestion with trypsin. Positive keratin staining, proved to be epithelial-derived cells. CONCLUSION: The incisor cervical epithelium cells were successfully cultured by enzymatic digestion.