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目的探讨百草枯(PQ)中毒患者外周血NOD样受体3(NLRP3)炎性体的变化。方法 PQ中毒患者78例分为死亡组(40例)和存活组(38例),收集体检正常者35例作为对照组。采用PCR方法检测三组外周血NLRP3、集合适配器调亡相关斑点样蛋白(ASC)和Caspase-1mRNA表达,ELISA方法检测IL-1β和IL-18水平。结果与对照组比较,存活组和死亡组的NLRP3、ASC、Caspase-1mRNA表达及IL-1β、IL-18水平升高(P<0.05或P<0.01),且死亡组各指标高于存活组(P<0.01)。结论 PQ中毒患者NLRP3炎性体升高,并且与患者死亡情况密切相关。
Objective To investigate the changes of peripheral blood NOD-like receptor 3 (NLRP3) inflammasome in patients with paraquat (PQ) poisoning. Methods 78 cases of PQ poisoning were divided into death group (40 cases) and survivor group (38 cases), 35 cases were collected as normal control group. PCR was used to detect the expression of NLRP3, ASC and Caspase-1 mRNA in the adapter, and IL-1β and IL-18 levels by ELISA. Results Compared with the control group, the expression of NLRP3, ASC, Caspase-1mRNA and the levels of IL-1β and IL-18 in survival group and death group were significantly higher than those in control group (P <0.05 or P <0.01) (P <0.01). Conclusions Patients with PQ poisoning have increased NLRP3 inflammasome and are closely related to the death of patients.