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为了寻求在较好地保持酶活力的同时解除L-天冬酰胺酶抗原性的方法,采用不同分子量的乙酸酐、右旋糖酐和单甲氧基聚乙二醇,作为修饰剂和不同的修饰方法对该酶进行了化学修饰。结果表明在保持酶活性和降低抗原性方面,大分子修饰剂右旋糖酐、单甲氧基聚乙二醇优于小分子乙酸酐,底物保护修饰优于直接修饰;活化PEG,优于活化PEG_1。在底物保护下的PEG,修饰酶其抗原性完全解除的同时,酶活力保持在30%以上。
In order to find a way to release the antigenicity of L-asparaginase while keeping the enzyme activity well, acetic anhydride, dextran and monomethoxy polyethylene glycol of different molecular weights were used as modifiers and different modification methods The enzyme has been chemically modified. The results showed that macromolecular modifiers dextran and monomethoxy polyethylene glycol were superior to small molecule acetic anhydride in preserving enzyme activity and reducing antigenicity, and the substrate protection modification was better than direct modification. Under the protection of substrate PEG, modified enzyme whose antigenicity is completely released, while maintaining the enzyme activity above 30%.